The Putidaredoxin Reductase-putidaredoxin Electron Transfer Complex: Theoretical and Experimental Study*
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چکیده
THE PUTIDAREDOXIN REDUCTASE-PUTIDAREDOXIN ELECTRON TRANSFER COMPLEX: THEORETICAL AND EXPERIMENTAL STUDY* Vadim Yu. Kuznetsov, Emek Blair, Patrick J. Farmer, Thomas L. Poulos, Amanda Pifferitti, and Irina F. Sevrioukova From the Departments of Molecular Biology and Biochemistry, Chemistry, and Physiology and Biophysics and the Program in Macromolecular Structure, University of California, Irvine, California 92612-3900 Running Title: Putidaredoxin Reductase-Putidaredoxin Interaction Address correspondence to: Irina F. Sevrioukova, Department of Molecular Biology and Biochemistry, 3205 McGaugh Hall, University of California, Irvine, CA 92697-3900, Tel. 949 824-1953; Fax. 949 824-3280; E-mail: [email protected]. Present address: Department of Physiology and Biophysics, University of California, Irvine, California 926997-4560.
منابع مشابه
Crystal structure of the putidaredoxin reductase x putidaredoxin electron transfer complex.
In the camphor monooxygenase system from Pseudomonas putida, the [2Fe-2S]-containing putidaredoxin (Pdx) shuttles electrons between the NADH-dependent putidaredoxin reductase (Pdr) and cytochrome P450(cam). The mechanism of the Pdr.Pdx redox couple has been investigated by a variety of techniques. One of the exceptions is x-ray crystallography as the native partners associate weakly and resist ...
متن کاملThe putidaredoxin reductase-putidaredoxin electron transfer complex: theoretical and experimental studies.
Interaction and electron transfer between putidaredoxin reductase (Pdr) and putidaredoxin (Pdx) from Pseudomonas putida was studied by molecular modeling, mutagenesis, and stopped flow techniques. Based on the crystal structures of Pdr and Pdx, a complex between the proteins was generated using computer graphics methods. In the model, Pdx is docked above the isoalloxazine ring of FAD of Pdr wit...
متن کاملCrystal Structure of the Putidaredoxin Reductase- Putidaredoxin Electron Transfer Complex
CRYSTAL STRUCTURE OF THE PUTIDAREDOXIN REDUCTASEPUTIDAREDOXIN ELECTRON TRANSFER COMPLEX Irina F. Sevrioukova, Thomas L. Poulos, and Inna Y. Churbanova⊥, From the Departments of Molecular Biology and Biochemistry, Chemistry and Pharmaceutical Sciences, University of California, Irvine, California 92697-3900 Running title: Crystal structure of the Pdr-Pdx complex Address correspondence to: Irina ...
متن کاملThe role of putidaredoxin and P450 cam in methylene hydroxylation.
Putidaredoxin, the iron sulfur protein from the P450,,,mediated methylene hydroxylase system of Pseudomonas putida, is shown to be essential both as an electron transfer agent and as an effector of P450,,, necessary for product formation. P450,,, is recognized to progress through four well defined states in an ordered reaction cycle of oxidized, and, with substrate, oxidized, reduced, and oxyge...
متن کاملElectron Transfer Proteins of Cytochrome P450 Systems
I . INTRODUCTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . , 2 9 SEQUENCE AND STRUCTURAL RELATIONSHIPS . . . . . . . . . . . . , 32 A. Adrenodoxin Reductase and Putidaredoxin Reductase . . . . . . . . . . . 32 H. Adrenodoxin and Putidaredoxin . , . . . . . . . . . . . . . . . . . . . . . 36 C. P450Reductase. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 8 I...
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تاریخ انتشار 2005